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Determination of Habayoside in Yanyan Tablets by HPLC
HPLC method was used to determine the content of abayanin in Yanyan tablets. Method: High performance liquid chromatography was used. Chromatography column: Agilent TC-C18 (250mm × 4.6mm, 5μm), mobile phase: linear gradient elution of acetonitrile-1% acetic acid solution, flow rate: 1.0ml · min-1, detection wavelength: 278nm, column temperature: 40 ° C. Results: Habayoside has a good linear relationship in the range of 1.0 ～ 50.0μg · ml-1 (r = 0.9997); the average sample recovery rate was 98.1%, RSD = 1.9% (n = 6). Conclusion: This method is simple and easy to perform, the results are accurate and reliable, and it can be applied to the quality control of Yanyan tablets.