Instruction Manual of Dalong Pipette

1. The new DRAGONMED PROLINE pipette: is a low-end manual pipette designed for simple sampling and dispensing. The operation of all pipettes in the PROLINE series is based on the principle of air displacement and uses disposable pipettes. The range of PROLINE pipettes ranges from 0.1ml to 5ml.

1.1 DRAGONMED PROLINE quantitative pipette

1.2 Compliance with standards: All DRAGONMED PROLINE pipettes have undergone sampling quality testing in accordance with Q / TFBM02-2002 and DIN 12650 standards. According to the requirements of Q / TFBM02-2002 and DIN12650, quality control includes the use of the manufacturer ’s original suction nozzle The pipette was gravity sampled with distilled water at 22 ° C.

1.3 DRAGONMED PROLINE pipettes cover all ranges of PROLINE pipettes and can be used with PROLINE pipettes. These separable disposable pipettes are made of natural colored polypropylene and are available with or without filter elements. The suction nozzle with filter is boxed and sterilized; the suction nozzle without filter is bulk or boxed and can be sterilized at high temperature and pressure (121 ° C, 0.11MPa)

Note: Pipettes are not equipped with pipettes, do not pipette!

2. package

The DRAGONMED PROLINE pipette packaging includes the following items:

l Pipette

l Calibration / disassembly tools

l Silicone oil

l Instructions

l Pipette suspension

l Suction nozzle

l Identification label

l Colored marking caps (except fixed pipettes)

l Filter element (> 10ml pipette)

l Performance certificate (according to Q / TFBM02-2002, DIN E-12650-7)

3. The pipette rack hook is installed for convenience and safety. When the pipette is not in use, it should be hung vertically on the rack hook. To install the pipette holder hook, please follow the instructions below:

3.1 Clean the shelf surface with alcohol

3.2 Peel off the protective paper on the adhesive

3.3 Press the rack hook along the edge of the shelf

3.4 Place the pipette on the rack hook

4 Pipette operation

4.1 Setting the range

The display window of the handle clearly shows the amount of liquid dispensed by the pipette. The pipetting volume is set by rotating the operation button clockwise or counterclockwise (adjustable pipette). When setting the range, please note

l Rotate to the desired range

l Clear numbers in the display window

l The set range is within the range of the pipette

Do not turn the button out of the measuring range, otherwise it will jam the mechanical device and damage the pipette.

4.2 Sealing and ejecting the suction nozzle

It is recommended to use the PROLINE pipette with a pipette. Before assembling the suction nozzle, make sure that the connecting piece of the suction nozzle is clean. Press the suction nozzle tightly on the pipette nozzle connection piece to ensure the seal is intact. The mark is that a clear seal is formed between the pipette nozzle and the black pipette connection piece.

Each pipette has a pipette ejector to eliminate the security threats posed by pollution.

Press the ejector down firmly to eject the nozzle. Take care to discard the suction nozzle into a suitable waste container.

4.3 2 in 1 pipette

PROLINE 5ml and 10ml fixed pipettes, and 0.5-10ml adjustable pipettes can be adapted to 10ml and 300ml pipettes. The pipette is equipped with two kinds of pipette ejection shafts at the same time. The factory is equipped with a shaft for 10ml pipettes. If using a 300ml pipette nozzle, please follow the steps below to replace the ejector shaft

l The nozzle ejector is pushed down to the end;

l Insert the pin of the gun dismantling tool into the middle part of the ejector rod and shaft of the suction nozzle, and dial the setting device

l Remove the nozzle and push out the shaft;

l Hold down the nozzle ejector downwards and insert the locking pin of the 300ml ejection shaft into the small hole on the ejection rod;

l Loosen the nozzle ejector, then press up and down to see if it moves freely

4.4 Protective filter

The PROLINE single-channel pipette (> 10ml) has a new type of pipette attachment that can be optionally equipped with a core changer. The filter element prevents liquid or aerosol from entering the pipette. There are two filter elements to choose from: standard type and reinforced type. The enhanced filter element can completely isolate liquid and aerosol. And the filter element does not affect the pipette calibration.

5. Pipetting technology

l Note that the pipette, pipette and liquid are at the same temperature

l Note that the suction nozzle is tightly fitted on the connecting piece

l The pipette stays upright when aspirating and the aspiration nozzle is only a few millimeters below the liquid

l Pre-wash the suction nozzle before aspirating, that is, aspirate and discharge the liquid 5 times. This is especially important when transferring viscous or dense liquids different from water

l Always use the thumb to control button movements to ensure consistency

5.1 Forward pipetting

This technology uses a blow-out function to ensure complete liquid transfer.

l Press the button to the first stop

l Submerge the suction nozzle 2-3mm below the liquid surface, and slowly release the button to return to the origin. Carefully lift the suction nozzle and stop on the container wall to remove excess liquid

l Press the button to the first stop point, the liquid will be discharged. After a short pause, continue to press the button to the second stop point (that is, blow out), this step will empty the suction nozzle to ensure accurate transfer of liquid

l Release the button to return to the original point. If the nozzle needs to be replaced, continue to pipette

5.2 Reverse pipetting

Using this technology will suck in more liquid than the set range, and the pipetting does not need the blow-out function, so that excess liquid remains in the suction nozzle. This technique is recommended when transferring highly viscous liquids, biologically active liquids, easily foamable liquids, or very small amounts of liquids.

l Press the button to the second stop point, submerge the suction nozzle 2-3mm below the liquid surface, and slowly release the button to return to the original point

l Carefully lift the suction nozzle and stop on the container wall to remove excess liquid

l Lightly press the button to the first stop point to discharge the set range, continue to hold the button, the liquid will remain in the suction nozzle without being transferred

l Excess liquid is discarded with the suction nozzle or returned to the original container

l If the storage pipette is not used, it is recommended to hang it vertically on the pipette holder. Install the pipette holder hook.

6. Performance testing and recalibration

Each pipette has been tested before leaving the factory and has a test certificate.

Note: Pipette performance is only guaranteed when used with DRAGONMED PROLINE pipette tips.

l Weighing must be at 20-25 ° C

6.1 Set the required pipetting volume

6.2 Tighten the suction nozzle carefully

6.3 Pre-wash the suction nozzle with distilled water, suction and discharge 5 times

6.4 Carefully aspirate the liquid and keep the pipette upright

6.5 Transfer distilled water to a weighed beaker, weigh with a balance of 1 / 100,000, repeat at least 5 times and record each result. Then calculate the range according to the density of water (20 ° C: 0.9982). The method is based on Q / TFBM02-2002, DIN 12650

6.6 Use the following formula to calculate the error value (F): F = | Accuracy | 2