Ten years ago, the resolution of the traditional optical microscope (Microscope) remained at 200 nm. After that, ultra-high-resolution imaging technology appeared, and now the optical microscope (Microscope) can achieve a resolution of 20 nm. To get a higher resolution, people have to resort to electron microscopes. Although the gap between these two imaging methods is getting smaller and smaller, it still exists. Biologists want to obtain the resolution of an electron microscope without fixing their samples.
In 2008, GFP discoverers and reformers were awarded the Nobel Prize in Chemistry, reflecting the importance of fluorescent proteins in cell biology research. As the understanding of the structure and function of fluorescent proteins has deepened, researchers have continuously modified to develop new fluorescent proteins suitable for new applications.
In 2012, it is expected that the family of fluorescent proteins will continue to expand, and more new fluorescent proteins will be born, used in ultra-high-resolution imaging, confocal microscopes, and even electron microscopes. Today, understanding how cells respond to external forces and how the stiffness of the cell ’s microenvironment affects cell biology has also become a topic of interest to researchers. Atomic force microscopy (AFM) uses a micro-cantilever to sense and magnify the force between the thin probe on the cantilever and the atoms of the sample under test, so as to achieve the purpose of detection, with atomic-level resolution. Since the atomic force microscope can observe both conductors and non-conductors, it makes up for the shortcomings of scanning tunneling microscopes. As atomic force microscope systems have become more user-friendly in recent years, some new methods and techniques may emerge this year to explore how forces inside and outside the cell regulate everything, from cell movement to differentiation potential.
In 2012, perhaps we will see more imaging technologies emerge, more fluorescent protein tools, and ultra-high-resolution imaging technologies enter new applications. In any case, every technological advancement in cell imaging methods will give us a deeper understanding of the world inside the cell.
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