This kit can only be used for scientific research, not for medical diagnosis. Rabbit thymosin β4 (Tβ4) quantitative detection kit (ELISA) user's Guide ã€Rabbit Thymosin β4 (Tβ4) Elisa Kit Kit Name】 Rabbit thymosin β4 (Tβ4) quantitative detection kit (ELISA) ã€Rabbit Thymosin β4 (Tβ4) Elisa Kit Kit Usage】 Quantitative detection of thymosin β4 (Tβ4) in rabbit serum, plasma, tissues and related fluid samples. [Rabbit Thymosin β4 (Tβ4) Elisa kit detection principle] This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). The standard and the sample to be tested are added to the transparent enzyme label coated plate of rabbit thymosin β4 (Tβ4) monoclonal antibody pre-coated. After incubation for a sufficient time, the unbound components are washed to remove, and then the enzyme label working solution is added. After incubation for a sufficient time, washing to remove unbound components. Substrates A and B are added in sequence, and the substrate (TMB) is converted into a blue product catalyzed by horseradish peroxidase (HRP), which becomes yellow under the action of acid. The concentration of (Tβ4) was positively correlated. The OD value was measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of rabbit thymosin β4 (Tβ4) in the sample was calculated. [Rabbit Thymosin β4 (Tβ4) Elisa Kit Kit Composition] 1 Enzyme coated plate 12 holes × 8 7 Developer A liquid 6mL 2 Standard 0.3mL × 6 tubes 8 Developer B liquid 6mL 3 20 times concentrated washing liquid 25mL 9 Stop solution 6mL 4 Sample diluent 6mL 10 Instructions 1 serving 5 Special diluent 6mL 11 Sealing film 2 sheets 6 Enzyme reagent 6mL 12 sealed bag 1 Remarks: The concentration of standard products (No. 1 → No. 6) is: 160, 80, 40, 20, 10, 5 mg / L. [Rabbit Thymosin β4 (Tβ4) Elisa kit required but not provided reagents and equipment] 1. 37 ℃ thermostat 2. Standard specification microplate reader 3. Precision pipettes and disposable tips 4. Distilled water 5. Disposable test tubes 6. Absorbent paper ã€Rabbits Thymosin β4 (Tβ4) Elisa Kit Operation Steps】 1. Preparation: Remove the reagent kit from the refrigerator and re-equilibrate at room temperature for 30 minutes. 2. Mixing solution: dilute the 20-fold concentrated washing solution with distilled water to the original one. 3. Add standard products and samples to be tested: take a sufficient number of enzyme-coated plates and fix them to the frame. Set up standard wells, sample wells to be tested and blank control wells, record the positions of each well in the standard wells. Add 50μL of standard product; first add 10μL of the sample to be tested, then add 40μL of sample diluent (that is, the sample is diluted 5 times); the blank control well is not added. 4. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes. 5. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each well with the washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the washing plate 4 times (you can also use the washing machine to press Instructions for washing the board). 6. Add enzyme-labeled working solution: add 50μL of enzyme-labeled working solution to each well, without adding blank control wells. 7. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes. 8. Plate washing: Discard the liquid, pat dry on the absorbent paper, fill each hole with the washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the plate washing 4 times (you can also use the washing machine to press Instructions for washing the board). 9. Color development: add 50μL of developer A solution to each well, and then add 50μL of developer B solution. Mix with a plate mixer for 30s (or shake gently by hand for 30s), and avoid color development at 37 ℃ for 15min . 10. Termination: Remove the enzyme labeling plate and add 50μL of stop solution to each well to stop the reaction (the color changes from blue to yellow). 11. Determination: Zero the blank holes, and within 15 minutes after termination, measure the absorbance (OD value) of each well with a wavelength of 450 nm. 12. Calculation: According to the concentration of the standard product and the corresponding OD value, calculate the linear regression equation of the standard curve, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. You can also use various application software to Calculation. The final concentration is the actual measured concentration times the dilution factor. [Rabbit thymosin β4 (Tβ4) Elisa kit sample requirements] 1. The sample cannot contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP). 2. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. 3. The sample should be fully centrifuged, without hemolysis and particles. ã€Precautions for rabbit thymosin β4 (Tβ4) Elisa kit】 1. The experiment is carried out in strict accordance with the instructions, and the result of the experiment must be determined by the reading of the microplate reader. 2. If the enzyme-labeled coated board is not used up after opening, it should be put into a sealed bag and added with desiccant immediately. 3. It is recommended that all standards, samples and blank controls be tested in duplicate, and the average value is taken to reduce the experimental error. 4. Keep in mind that the sample has been diluted 5 times. The calculation result is multiplied by 5 to obtain the actual concentration of the sample. 5. The quantitative range of this kit is 5-160mg / L. If it exceeds this range, it is calculated from the extension of the standard curve. It is not used as an accurate quantitative result. ), Multiplied by the total dilution factor is the final concentration of the sample. 6. If the color is too light, the substrate incubation time can be extended properly. 7. In order to avoid cross-contamination, the tips, samples and blank controls should be replaced with a new one for each addition; the common components such as enzyme working solution, sample diluent and substrate should be cantilevered, and they should not touch the microwells. ; Do not reuse the sealing film. 8. The kits are used within the warranty period, and different batches of reagents should not be mixed. 9. Substrate B is sensitive to light and avoid prolonged exposure to light. [Rabbit thymosin β4 (Tβ4) Elisa kit operating procedures summary] Prepare reagents, samples and standards Add prepared samples and standards, and react at 37 ℃ for 30 minutes Wash the plate 4 times, add the enzyme reagent, and react at 37 ℃ for 30 minutes Wash the plate 4 times, add color developing solutions A and B, and develop at 37 ℃ for 15 minutes Add stop solution Read OD value within 15 minutes Calculation [Rabbit Thymosin β4 (Tβ4) Elisa kit detection range] 5mg / L – 160mg / L ã€Rabbit Thymosin β4 (Tβ4) Elisa Kit Specifications】 96T / box ã€Storage of Rabbit Thymosin β4 (Tβ4) Elisa Kit】 Store at 2-8 ℃, protected from light and moisture. [Rabbit thymosin β4 (Tβ4) Elisa kit expiration date] 6 months Garbage truck is used for municipal sanitation department dedicated to deliver a kind of special vehicles of various kinds of rubbish. ISUZU Garbage Truck,Garbage Compactor Truck,Hook Lift Garbage Truck,Small Garbage Truck,Waste Management Truck,Hydraulic Lift Garbage Truck,Garbage Transporting Truck CLW GROUP TRUCK , https://www.clwgrouptruck.net
Garbage truck,dump cart, garbage truck is applied to municipal sanitation garbage trucks carrying a kind of special vehicles of various kinds of rubbish. Garbage truck types are divided into: compression garbage truck, dump garbage truck, garbage truck swing arm, self loader garbage truck, hook lift garbage truck (trunk detachable type garbage truck) sealed garbage trucks, hang barrel type garbage truck (since the loading and unloading garbage truck),Ro on ro off garbage truck. Mainly includes: the dongfeng, changan, FOTON, JAC, ISUZU, JMC, Forland series; garbage trucks are divided into: dongfeng xiaobawang garbage truck, dongfeng dolika garbage truck , dongfeng light duty garbage truck truck, dongfeng long head garbage truck, dongfeng 145 garbage truck, dongfeng 153 garbage truck, changan garbage trucks, FOTON, jianghuai JAC garbage trucks, ISUZU garbage trucks ,JMC Garbage truck, Forland garbage truck and so on. Garbage truck is mainly used in municipal sanitation, large factories and mines, usually used to transport all kinds of rubbish, apply to the transportation community living garbage, and can be loaded garbage compression, crushing, its density, shrinkage, greatly improve the efficiency of garbage collection and transportation. New truck has reliable quality, low fault rate and easy maintenance, low operation cost etc.
CLW GROUP TRUCK Garbage truck is mainly carry living garbage, can also transport dust, sand, stone, soil and other bulk materials, can also be in mine coal chienchung send ore or coal.
Garbage trucks function introduction: bucket can lift commit suicide by hanging, a swing arm 60 s work cycle time, the characteristics of the vehicle is rubbish bucket is separated from the body, can achieve a car with multiple trash bucket joint use, circulation transportation, fully improve the transportation capacity of vehicle, especially suitable for short-distance transport, loading, such as the sanitation department of town and cleaning, transportation, etc.
CLW GROUP TRUCK Compression garbage truck,Compactor Garbage truck by adopting the technology of mechanical and electrical integration of hydraulic, USES the automatic control system of machine, electricity, Hydraulic , filling machine, pushing shovel special device, such as the garbage load, crush, or crushed, strong loading, finally will waste compaction into cars, to destination automatically push unloading. Its outstanding characteristic: garbage collection way is simple, efficient, repeated compression and creep compression and high compression ratio, large loading quality, automation, power performance, environmental protection, high utilization efficiency of the vehicle.
Taken the compression type garbage truck abstract: compression type garbage truck is mainly through garbage load, the loading device, pushing shovel, hydraulic system, a special device such as implementation rubbish into, crush, or crushed new green the compression type garbage truck with garbage collection convenient, efficient and can automatically compressed garbage repeatedly, and creep compression, high compression ratio, large loading capacity, operation automation, sealing good characteristic.
Taken the compression type garbage truck is fully sealed type, compression in the process of sewage directly into the sewage, completely solve the secondary pollution in the process of waste transportation, key parts imported components.
Painted with the bucket hoisting frame, can be used the national general iron barrel or plastic barrels, strong loading, the trash shoved into the carriage and compaction and shirk.
type :Swing arm garbage truck
CLW GROUP TRUCK Swing arm type garbage truck configuration of the hydraulic cylinder is 1680 mm / 125 mm diameter; Swing arm stroke is: the empty flat a is 3740 mm, vertical set up working conditions as high as 3890 millimeters, multi-channel valve for the hydraulic parts factory production, function: urban garbage collection, transfer, has simple structure, flexible operation, reliable performance, high transfer efficiency (a car can match multiple trash bucket), etc.
Garbage truck products are mainly used for carrying garbage, can also transport dust, sand, stone, soil and other bulk building material, also can be in mining or coal chienchung transport ore or coal.
Sealed garbage truck
Hermetic garbage truck is mainly composed of automobile chassis, cabinets, open the door, the lifting mechanism and the electro-hydraulic control system, etc, is a new kind of machine, electricity, liquid integration fully sealed structure of the waste transportation vehicles, it not only can be used with the newly-modified LSY series of form a complete set of tips, also can be widely used in urban residents living area, industrial, commercial districts, schools, parks, scenic places such as garbage transport work.
Docking garbage truck
Docking garbage truck is garbage compression station special garbage truck, with hydraulic back cover can be opened, after loading compression good garbage block from behind, increased the volume of waste. This car is sealed, also can prevent the secondary pollution in the process of transportation.
Compression garbage truck
Compression type garbage truck used mechanical and electrical integration of liquid technology, main compartments, filling machine, pushing through garbage shovel, hydraulic system and so on special device, with automatic control system of machine, electricity, liquid, by car, fill in and pushing shovel device such as a dedicated and realize the rubbish into, crush, or crushed, strong packing, put the rubbish into carriages, compaction, and discharge function. Its main characteristic is garbage collection way is simple, efficient, with functions of automatic compression and creep compression, compression ratio, high loading quality, automation, power performance, environmental protection, high utilization efficiency of the vehicle, sealing good characteristic.
Food waste garbage truck
Food waste garbage truck is also called the Rest meal garbage truck, kitchen waste garbage trucks or waste food garbage truck, eat hutch garbage truck is one of the garbage truck, is used to collect and transport waste, food waste (swill) and special vehicles of city sludge. With the development of catering industry in our country, our country nearly 350 existing hotels, restaurants, a surprisingly high number of eat hutch garbage produced every day, its diverse collection transport way. To eat hutch garbage objects of eat hutch garbage truck is also growing fast.
Side Loader garbage truck
Side Loader garbage truck also known as the loading and unloading garbage truck, hang garbage bin garbage truck, it is mainly used for the sanitation department of municipal and large factories and mines carry a variety of waste, can also transport dust, sand, stone, soil and other bulk materials, can also transport in the mines or mine ore or coal. The sealed garbage load, hydraulic system, the operating system. Lifting system of oil cylinder (2) garbage compartments can hang down, working on the next cycle time 50 s or less.
Multifunctional garbage truck
Other garbage truck vehicles
Van body material: garbage trucks can be used (carbon steel, stainless steel, internal adopt epoxy anticorrosive processing)
Use scope: garbage trucks are widely used and city streets, garbage disposal have dump function, hydraulic operation, the dumping of convenience.
Composition of garbage truck
Garbage truck has compression garbage truck, garbage truck swing arm, hanging barrels of garbage truck (since the loading and unloading garbage truck), sealed garbage trucks, docking garbage truck etc. Different configuration.
Of compressed garbage truck: consists of chassis, sealed garbage load, hydraulic system, as a system. The vehicle is fully sealed type, compression, dumping, all sewage into the sewage compartments in the process of compression.
Swing arm of garbage truck: consists of chassis, hydraulic lifting assembly, rubbish bucket, by about two arms shipment unified national swing arm type rubbish bucket, can be more than a car, with the function of dump, to achieve the safe and stable, reliable in performance.
Hanging barrels of garbage truck: chassis, load's total, automatic feeding device, hydraulic system, a system, etc.
Seal of garbage truck: by car chassis, cabinets, open the door, the lifting mechanism, electro-hydraulic control system, etc.
Docking garbage truck,connection garbage truck: consists of chassis, hydraulic control system, waste compartments, as a system.